Chemistry is a crucial discipline in life. In chemistry processes, you will make items using chemicals. There are various Chemistry courses provided at various institutions of higher learning.
It is common for you to find chemistry people separating matter in a certain chemical mixture. HPLC is a method used in chemistry to separate various elements of a varying nature in a particular mixture. In HPLC, chromatography principles are applied to separate. The identification and quantifying of the elements in a specific mixture is made possible by spectroscopy. Silica gel is preferred as a stationary phase in HPLC columns because it does not react with the mobile phases used in component separation. You will then need a computer to analyze your data. It is possible to separate components in a mixture because they have different properties.
The five section you will get on a HPLC column are the computer, a pump, detector, column, and the reservoir. You should be aware of the fact that the reservoir in a liquid chromatography stores solvent. Some of the conditions that affect the retention time are the nature of the solvent, the composition of the stationary phase, the temperature level, as well as the amount of pressure in the column. High performance liquid chromatography column need UV light which is absorbed by different components at different wavelengths for easy detection.
There are several kinds of HPLC columns used to separate components in a mixture. The type of high performance liquid chromatography column you use will depend on the nature of the elements you are separating. Some types of HPLC columns are the size exclusion column, the reverse phase column, the normal phase column, and the ion exchange column. The normal phase chromatography columns are the ones whose stationary phase is more polar while the mobile phase is less polar.
You have to use a non-polar stationary phase in liquid chromatography and a polar solvent in separation of compounds in a reverse phase HPLC column. There are various types of non-polar stationary phases is use such as C8 and HPLC column C18 bonded hydrocarbons. Commonly used components for the mobile phase are the water-acetonitrile mixture and the water methanol.
If an element to be separated in easy to ionize then the best type of HPLC column to use is the ion exchange chromatography column. Stationary phase materials has positive or negative charge. The mobile phase in this types of HPLC column is supposed to be a polar material. You have to make sure that the components you are trying to separate can be separated by ionic exchange so that you use the ion exchange HPLC columns.
In the size exclusion HPLC column, there is a porous stationary phase which makes it possible to separate certain elements depending on their size. You are supposed to use a mixture of polymers to get a porous stationary material.
Browse more details at this link: https://en.wikipedia.org/wiki/Monolithic_HPLC_column